As a generous estimate, you might consider 300 W (at 120 Volts) per Spectramax reader, plus another 300 W for the Computer. es •. Self Service Available. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the. If you do not have an iLab account you must first make one: Molecular Devices SpectraMax M3 Multi-Mode Microplate Reader is a dual-monochromator, multi-detection microplate reader with full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. SpectraMax M3, M4, M5, and M5 Multi-Mode Microplate Readers Read the Cuvette 1. FlexStation™ III. Description 2 SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual. 2. SpectraMax® M4. Plasma Tau was determined by high-performance liquid chromatography (Shimadzu ®, model LC 10AD) using a Shimadzu model RF 535 fluorescence detector. 6 0. Here is the problem: Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The CellTiter-Glo assay from Promega uses luciferase. The SpectraMax i3x Microplate Reader possesses the five detection modes preferred by the majority of users: The SpectroMax i3x Microplate Reader is the standard for UV/ Visible multimode reader absorbance, providing ultrafast, full spectral range dectection for cuvettes as well as 96- and 384-well microplates. Ltd. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. This monochromator-based instrument allows users to select optimal wavelengths for any chosen fluorophore without the need to purchase additional filter sets. Ltd. Flurescence intensities were read using a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA). Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. Molecular Devices Spectramax M5e Multi Mode Microplate Reader; Related products. Choose from a three- (M3), four-. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. Plate chamber design of the SpectraMax category 1 microplate readers. Manufacturer: Molecular Devices; Model: SpectraMax; The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. SpectraMax M5 reader settings are shown. For readers without a cuvette port, these options are not shown in the software. Molecular Devices Corporation. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. SoftMax Pro. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. The calculated LOQ values were 0. anisotropy was measured on SpectraMax M5 microplate reader with 485 nm excitation and 535 nm emission after 60 min incubation at room temperature. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. 8 µg pNF-κB-After 3 h of incubation at 37 °C, the medium was gently removed, and a 150-μL aliquot of DMSO was added to each well. AquaMax® 4000. Background fluorescence has been subtracted. This application note describes the use of the SPECTRAmax ® GEMINI XS from Molecular Devices to study the performance of the LIVE/DEAD ® Viability/Cytoxicity Assay Kit for animal cells. They were allowed to attach and grow overnight in a 37°C, 5% CO 2 incubator. . Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. analysis, controls the SpectraMax Multi-Mode Microplate Readers. M3 M4 M5 M5e LM1 v<nnn>. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Published Dec 16, 2020 | Updated Dec 16, 2020. 306 plate air cal. com. The purpose of this procedure is to set instructions in the proper use and handling of the Molecular Devices M5 Plate Reader. Add to Cart. Cavicide (Warehouse, Cat # 79300360). spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Tunable wavelength selection / monochromator. The CellTiter-Glo assay from Promega uses luciferase. 00 USD. AquaMax® 4000. You can use either. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384. Analyst, M5, Flex 3, and SpectraMax L readers work differently. Spectramax M5 microplate reader: Molecular Devices: N/A:. E Models. Design of genetic materials Sequence of β-gal siRNA was designed (5'-CA GUU GCG CAG CCU GAA UGU U3', 5'AA CAU UCA GGC UGC GCA ACU G-3') and nonsense siRNA ( Silencer ® negative control siRNA). SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices. Then, the fluorescence change rates (F 0 − F)/F 0 were calculated, and the selectivity histogram. Figure 1. Turn on the microplate reader. The dual monochromator optics allow the widest range of applications to be. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD5 reader. Or scan up to 384 wells at any time to determine the best assay conditions. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. 1. SpectraMax® Plus 384 Absorbance Plate Reader, Molecular Devices. SpectraMax M3 M4 M5 M5e SpectraTest FL1 revA. View the Molecular Devices website. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. To quantify the A1AT in rat serum, standard curves were drawn for nA1AT, rA1AT-WT and rA1AT mutants, respectively. Add to Cart. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplateinstalled if you are using a SpectraMax ABS microplate reader **SoftMax Pro Software version 7. Qualify the fluorescence performance of the SpectraMax . or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. Note: this assay can also be detected using the luminescence mode of SpectraMax M5/M5 e and FlexStation 3multi-mode microplate readers. Bookings are free, but you will need to use iLab to book. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. 5 Dual-luciferase reporter assay. Figure 1. The SpectraMax® ABS Plus Microplate Reader can run . For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. 0 . 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The chamber contains springs that position the cuvette in the proper alignment for a read. SpectraMax M2, M2e; SpectraMax M3, M4, M5, M5e; FilterMax™ Multi-Mode Detection Platform. Figure 1. Trilevel commercial control reagents (StanbioThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. Depending on which. Pick the best speed, sensitivity, and price for your needs. 315 can't find zero order. Example: [Drawer]. 7 1. Cables. Fluorescence was measured (Ex 530/Em 590) in a SpectraMax M5 microplate reader. 04 to 3. While they play an important role in generating data from a wide range of. Softmax Pro GxP Software 6. Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Small, unbound molecules rotate rapidly in. It is important to determine the excitation and emission wavelengths that provide the best dynamic range for the assay, since these may differ somewhat from those used with filter-based plate readers. How do I set up Hellma Cuvette validation set recertification? Send Hellma cuvette standards to Hellma. Figure 1. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Step 1. Maximum signal with lowest background. es •. Plate Shaking. • SpectraMax M5 Multi-Mode Microplate Reader Figure 2. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. . SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. FlexStation™ II. IMAP FP PDE Evaluation Kit (Molecular Devices P/N R8175)The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Most papers they cultured bacteria to OD600>0. Insert the cuvette into the chamber, making sure that the clear sides are to the left and right (facing the instrument). All reagents were brought to room temperature before use. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. After 16 hr of treatment, cell viability was measured by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s. Supplier: Molecular Devices. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. • SpectraMax Mini Multi-Mode Microplate Reader (AccuBlue HiRange only) • SpectraMax® M2/M2e/M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate Reader • FlexStation® 3 Multi-Mode Microplate Reader • Gemini™ EM/XPS Fluorescence Microplate Readers • FilterMax™ F3/F5 Multi-Mode. p. 00 USD. Filterless readers provide consistent results with steady temperature regulation. The drawing shows a standard microplate of 15 mm height covered with a lid. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. Bottom-reading will also give you higher RFU's (and the appearance of high sensitivity) because the polystyrene will fluoresce a bit. metrically at 540 nm using a microplate reader (SpectraMax 384 Plus, Molecular Devices Corp. Has anyone had problems with the built-in computer of the Spectramax ID3. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. SpectraMax Gemini EM Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini EM Reader, users never have to worry about not having the right set of filters. 2. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. Compact and tunable absorbance microplate readers DATA SHEET. 0 out of 5 $ 2,499 $ 1,999. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. The system also gives users the flexibility to choose wavelengths between 200 nm and 1000 nm. Choose from a three- (M3), four- (M4), or The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. 2. book Page 7 Wednesday, July 7, 2010 2:09 PM. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. Five. In SOFTmax PRO, set up a plate section for an excitation scan with no cutoff filter and use the medium PMT setting. Introduction. "With the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers, we now offer a full range of multi-mode detection capabilities within our SpectraMax® product line," said Andy Boorn, President of MDS Analytical Technologies. Maximum signal with lowest background. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. After 24 h drug treatment, cells were lysed by RIPA buffer (Life Technologies Inc, US). The cells were incubated with compounds at the indicated doses for 1 h. RAW 264. Standard 6-, 12-, 24-, 48-, 96- and 384-well microplates. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance,. 0 out of. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. The SpectraMax M5e Multi. Equipped with scanning, shaking and kinetics modules. Figure 4: Transcreener ADP. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Compatible automation solutions for SpectraMax Readers. D. Expanded wavelength coverage. edu) to be added as a trained user in iLab. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideMolecular Devices SpectraMax M5 Multi-mode Plate Reader 6. "With this complete portfolio of multi-mode configurations, we. Molecular Devices ThermoMax Microplate Reader COMPLETE w WARRANTY $1,750. Access this by going to Help >. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. 54 1. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. SpectraMax M3, M4, M5 and M5e Multi-Mode Microplate Readers User Guide Fluorescence Microplate Reader Comparison Chart. Here the delta mP was 336, and Z’ factor was 0. , Sunny-vale, CA). Molecular Devices SpectraMax® series microplate readers. 2 only). A control experiment of the activity of the proposed enzyme/ZIF-8A particles without peptides against protease digestion was also performed. 6-384. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Add to Cart. Detailed specifications of each model reader are reported in Section 7, “Specifications”. 5 Compatible microplate readers FP Plate Reader SpectraMax® M5/M5e Multi-Mode Microplate ReaderSpectraMax M5 Multi-Mode Microplate Reader; Method. Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. 1. Five preferred detection modes. SoftMax Pro 6 User Guide is a comprehensive manual for the latest version of the software that allows you to control and analyze data from microplate readers. 1. The Gemini EM can sample a variety of formats, from 6- to 384-well microplates, in different modes, such as endpoint, spectral scan, kinetic, and well scan. Italic and boldface type are used for emphasis. book Page 1 Wednesday, July 7, 2010 2:09 PM The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 7 1. These lab workhorses are used in drug discovery, research, bioassay validation, and. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. SpectraMax® Microplate Readers and SoftMax® Pro Software. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. A serial dilution of IgG standards was performed, using XP Media/L-glutamine as the diluent, to concentrations from 2. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. SpectraMax M Series multi-mode microplates are configurable readers with triple. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Optical absorbance. Our absorbance plate readers feature our PathCheck Sensor technology and. Temperature variation ± 0. 67. AlphaScreen. ,. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. ZERO BIAS - scores, article reviews, protocol conditions and moreThe absorbance was measured at 450–650 nm using a SpectraMAX M5 microplate reader (Molecular Devices). The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Sale price $16,495. When a SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. 5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuide SpectraMax® M5 Multi-Mode Microplate Reader (Molecular Devices P/N #M5) SpectraMax® Gemini™ EM Microplate Reader (Molecular Devices P/N #EM) Methods Instrument and protocol setup. 04 to 3. 3 1. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. 1 & 7. The introduction of the Molecular Devices’ SPECTRAmax PLUS microplate spectrophotometer marks the first time that photometric measurements made in a microplate format can be automatically normalized such that the resultant values are identical to those obtained with the corresponding solutions in a standard 1 cm. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. Example: [Drawer]. . No additional manuals are available. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. Readings were taken using a UV–Vis Spectramax M5 ® microplate reader (Molecular Device, USA). 2 Figure 3 shows the same assay plate read on the Analyst HT multi-mode reader. reader in the visible range between 405–750 nm with NIST and NMI. When a feature or capability applies to only certain readers, this exception is noted. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. Synergy H1 Hybrid Multi-Mode Reader Synergy™ H1 is a configurable multi-mode microplate reader. temperature and fluorescence was measured at Ex/Em 490/585 nm using a Molecular Devices SpectraMax M5 microplate reader. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. SpectraMax Plus 384 (Molecular Devices) [4 units] UV and visible absorbance monochromator-based microplate reader SpectraMax M5 (Molecular Devices) [2 units] multi-label monochromator and filter-based microplate reader for UV and visible absorbance, fluorescence intensity, time-resolved fluorescence, fluorescence. Products : SpectraMax M2/M2e reader, SpectraMax M3 reader, SpectraMax M4 reader, SoftMax Pro Software, SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax M5/M5e reader. Instrument settings are shown in Figure 3. 63 1. Also refer to this Multimode Reader Comparison Chart . Learn how to operate the VersaMax, SpectraMax 340PC384 and SpectraMax 190 Plus384 microplate readers, which offer fast and accurate absorbance measurements for a variety of assays. 40266 Email: drugdiscoverytech@lifetech. view more . The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based assays (M2e). To block unwanted residual excitation light and minimize background interference, a 570 nm. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). The BioTek Synergy H1 multimode microplate reader is modular and upgradable to include variable-bandwidth monochromator optics for fluorescence excitation and emission wavelength selection, plus luminescence and UV-Vis absorbance. The tunable SpectraMax M5e is a monochromator-based reader developed in collaboration with Cisbio international and is certified to run HTRF® assays. Microplate Readers Biotek ELx808 Microplate Reader Absorbance. Absorbance was read on a Spectramax M5 Microplate reader at 450 nm. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a. (Beijing, China) and a BCA protein assay kit from Beijing Solarbio Technology Co. MOLECULAR DEVICES SpectraMax M5 Microplate Reader For Sale. If you do not have an iLab account you must first make one: would like to show you a description here but the site won’t allow us. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Bottom-reading is usually recommended for cell-based assay with adherent cells. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. SpectraMax M5eは、宇宙に打ち上げられた世界初のマイクロプレートリーダーです！ NanoRacksとモレキュラーデバイスとのパートナーシップ、そしてSpaceX Commercial Resupply Services MissonでSpectraMax M5eを打ち上げた取り組みの詳細については、こちらをクリックしてください。Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. 6% for the on-board optics (monochromators). Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. microplate reader. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. We would like to show you a description here but the site won’t allow us. ATG provides repair, maintenance, and contract services for laboratory research and bioprocess instruments. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Then, after H 2 O 2 was added, the fluorescence was measured for each time period at 488 nm (excitation) and 525 nm (emission) wavelengths on a SpectraMax M5 microplate reader. Five preferred detection modes. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. Microplate formats. The guide also covers the software features, the specifications, and the accessories of the systems. M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence. 05% Tween 20 using a Nunc ELISA plate washer. Selection of Water Constant in PathCheck settings. The detection of lactate dehydrogenase releaseBriefly, algae were harvested, centrifuged, and re-suspended with an equal volume of methanol at the end of the experiment. both cuvette-based and microplate reader applications on the same instrument. Description. Add to Cart. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Taurine ≥99 % was used as standard (Sigma-Aldrich ®,. Molecular Devices. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. 7 cells and HEK293T cells were seeded at the density of 2 × 105 cells per well in a 24-well plate overnight. KEY FEATURES • Five. With only a few product lines that we provide service for, our engineers have unrivaled expertise in the SpectraMax® microplate readers. Click SoftMax Pro Software User Guide. VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® Plus 384 Absorbance Microplate Reader; SpectraMax® L (software version 5. 314 low lamp level warning. Recertification is recommended every two years. The SpectraMax M5 features a triple-mode cuvette port and a single-read command capability for experiments requiring multiple detection parameters. 7 cells were co-transfected with 0. Choose from a three- (M3), four- (M4), orWe would like to show you a description here but the site won’t allow us. SpectraMax 340PC384 Microplate Reader A versatile 96- and 384-well format absorbance reader let the monochromator tune to that exact wavelength. SoftMax® Pro Software is the most published microplate reader control and data analysis software in the industry. Cell viability was assayed by addition of BacTiter-Glo ™ bioluminescence reagent according to the manufacturer’s protocol (Promega) and luminescence measured using a Spectramax M5 microplate reader (Molecular Devices). Measurements of fluorescence and fluorescence polarization were performed on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA) with the excitation and emission wavelengths 544 nm and 590 nm, respectively. 2. Self Service Available. Molecular Devices Spectramax M5 Microplate Reader. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. 73. In brief, RAW 264. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. " The SpectraMax M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. However, when a specific probe is not available, or it causes off-target effects on the. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. Biotek FLx800 Fluorescence Microplate Reader; Related products-20%. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a. flexibility of monochromator-based systems. Bio-Tek BioTek uQuant Microplate Reader Complete w WARRANTY $3,450. ) by nonlinear regression using. The. com A. The absorbance was recorded using a SpectraMax M5 microplate reader (Molecular Devices). View the Molecular Devices website. Microplate Reader Shelf. ATP standard curves read on SpectraMax microplate readers. Bioz Stars score: 86/100, based on 1 PubMed citations. Comprehensive software and validation Comprehensive data analysis and GxP solutions SoftMax. After two more rinses with Hank’s solution, fluorescence intensity of the red/green ratio was determined on a Spectramax M5 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at an excitation of 490 nm and emission of 530 nm (green fluorescent monomers) and 590 nm (red fluorescent aggregates) respectively. on the SpectraMax Multi-Mode Microplate Readers by issuing a single. Molecular Devices SpectraMax™ Plus 384 Microplate Reader (s) with One-Year Warranty $9,000. Molecular Devices FlexStation™ 3 Multi-Mode Microplate Reader (Recent Model) with One Year Warranty (Svc Contract Available) Molecular Devices SpectraMax™ M5e Microplate Reader with 90 Day Depot Warranty (Svc Contract Available). 2. 96-1536. Molecular Devices LMax II Luminometer Luminescence Microplate Reader w WARRANTY $2,450. Microplate Reader, MultimodeProducts : SpectraMax M2/M2e reader, SpectraMax Plus 384 reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, SpectraMax M3 reader. book Page 1 Wednesday, October 12, 2011 4:56 PMSpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. The following document is intended to demonstrate setup of this instrument. Learn how to set up protocols, customize reports, export results, and more with this PDF document. As the ratio of ADP:ATP increases, the proportion of bound tracer vs. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. As readout, fluorescence was measured at Ex/Em = 540/590 nm using a SpectraMax M5 microplate reader with SoftMax Pro v7 (Molecular devices) after 4–6 h of incubation in normal cell-culture medium. The SpectraMax® ABS Plus Microplate Reader can run . 72 MB. : 2 Oct 12 Page 2 of 10 Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. Download PDF. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Figure 1. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. IMAP assays are based on binding of. RAW 264. Thank you!. Plates are used to validate optical performance of SpectraMax M3, M4, and M5/M5e readers. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. SpectraMax® M5. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. (The i3x and Paradigm readers require the. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. SoftMax Pro Software has been integrated by many leading robotics andFor readers without a cuvette port, simply check the box next to ‘PathCheck’. Table of Contents. Designed to provide the simplicity, flexibility and power required for advanced data analysis. The optical density (OD) was measured at 450 nm with a SpectraMax M5 microplate reader (Molecular Devices). book Page 1 Wednesday, July 7, 2010 2:09 PMThe SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers.